Extracts of Different Polarity of Daphne laureola L. as Valuable Source of Antioxidant and Neuroprotective Compounds

In this study, Daphne laureola L., a European–Mediterranean species, was investigated for its antioxidant properties using different in vitro bioassays, namely 2,2?-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Activity Power (FRAP), and ?-carotene bleaching assays. The acetylcholinesterase (AChE) butyrylcholinesterase (BChE) inhibitory activity Ellman’s method also examined. aerial parts of D. were subjected to exhaustive subsequent macerations with solvents at polarities, such as methanol, dichloromethane, n-hexane. Dichloromethane extract the most promising DPPH FRAP tests IC50 values 32.2 ?g/mL 71.5 ?M Fe(II)/g, respectively. Methanol showed inhibition lipid peroxidation evaluated by test an value 6.6 after 30 min incubation. This active against AChE (IC50 56.9 ?g/mL). An interesting result obtained BChE n-hexane extract, 49.7 ?g/mL. conclusion, results suggest that may provide substantial source phytochemicals, which act natural antioxidants inhibitors, be beneficial treatment Alzheimer disease.